supersensitive BPA ELISA Kit過敏雙酚A試劑盒
Bisphenol A (BPA), principal raw material for polycarbonate and epoxy resins, is suspected as one of the endocrine disrupting chemicals.
GC/MS, generally employed methods for quantitative BPA analysis, requires expensive instrumentation as well as complex and time-consuming extraction process with hazardous organic solvent.
This test kit detects BPA in environment or in vitro with high sensitivity with the aid of solid phase extraction.
Features
• The quantitative analysis ranges from 0.05-10μg/L(ppb), sensitive enough to detect BPA in a specimen from river water, etc..
• A simple solid phase extraction is available to measure samples with lower concentration.
• The total time for measurement is only 2.5 hours.
• BPA monoclonal antibody binds exclusively with BPA and does not show cross-reaction with other chemicals with similar structures.
• The amount of harmful solvent can be minimized.
Standard Curve of BPA
Cross-Reactivity Pattern
Compounds Cross Reactivity(%)
Bisphenol A (BPA) 100
Diethylhexylphthalate (DEHP) <0.05
Nonylphenol (NP) 0.19
17β-Estradiol (E2) <0.05
Estrone (E1) <0.05
Linear alkylbenzene sulfonate (LAS) <0.05
Alkylphenol Ethoxylate (APE) <0.05
Alkyl Ethoxylate (AE) <0.05
Humic acid Na <0.05
.
Kit Format
Kit Format Comment
Microplate 1 plate (96 wells)& reagents Needs a microplate reader (450nm)
For multiple and simultaneous measurement
Simple Solid Phase Extraction (example)
• Adjust the pH of the sample to 3.5 with 1M HCl.
• Wash out C18 solid phase cartridge with MeOH and distilled water; then pour the sample through the cartridge.
• Rinse the cartridge with water, then elute the mixture of aceton and MeOH at the ratio of 1:1.
• Dry the sample with nitrogen gas, then adjust MeOH concentration at 10% for measurement.
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