Total Estrogen (E1/E2/E3) ELISA Kit 雌激素試劑盒
Total Estrogen (E1/E2/E3) ELISA Kit
• ES monoclonal antibody binds exclusively with E1, E2 and E3, and does not show cross-reaction with other chemicals of similar structures. A monoclonal antibody is uniform in quality, generating very little lot-to-lot variation.
• The quantitative analysis ranges from 0.1μg/L to 3μg/L (ppb).
• The ELISA measurement is highly reproducible; the coefficient of variation (CV) is mostly under 10%.
• The assay requires less amount of harmful solvent than instrument analyses.
• With ease of handling, the total time for measurement is only 2.5 hours.
• The kit, a 96-well microplate format, enables simultaneous measurement of multiple samples at more reasonable cost.
Chemical Structures of Targets
Standard Curves
Pretreatment Methods
See here.
Test Data
Recovery Test using River Water (n=4)
ES ELISA Kit
Unspiked
Determination (ng/L)
12.4
SD (%)
6.5
Spiked with 2ng/L E2
Determination (ng/L)
14.4
SD (%)
9.2
Recovery (%) (n=4)
100.0
Comparison with LC/MS/MS
Comparison with LC/MS/MS
E1+E2 (ng/L)
Sample LC/MS/MS ES ELISA Kit
A 2.08 3.75(1.8)
B 4.25 5.36(1.3)
Figures in parentheses indicate relative value to LC/MS/MS data.
Cross-reactivity Pattern
Estrogens
Compound % Reactivity
ES ELISA Kit
Estron (E1) 87.0
2-methoxy E1 <0.03
E1-3-sulfate <0.03
17β-Estradiol (E2) 100.0
16-keto E2 118.0
2-methoxy E2 0.2
E2-17-glucronide 5.0
E2-3-glucronide <0.03
E2-3-sulfate-17-glucronide 0.5
Ethynylestradiol 0.7
Estriol (E3) 55.0
16-epi-E3 129.0
E3-16-glucronide 48.0
Other hormones
Compound % Reactivity
ES ELISA Kit
cis -Androsterone <0.03
trans -Androsterone <0.03
Cholesterol <0.03
Dehydroisoandrosterone <0.03
5α-Dihydroteststerone <0.03
Hydrocortisone <0.03
Pregnenolone <0.03
Teststerone <0.03
Kit Format
Kit Format Comment
Microplate 1 plate (96 wells)& reagents Needs a microplate reader (450nm)
For multiple and simultaneous measurement
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